Composite

Part:BBa_K736002:Design

Designed by: Lethbridge iGEM High School Team   Group: iGEM12_Lethbridge_Canada   (2012-06-16)

Constitutive Promoter with MLC-binding-site-regulated enhanced RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Red fluorescent protein is an easily detectable reporter, allowing for easy proof of principle.


Source

BBa_K736000 and BBa_I13507 were assembled by the Lethbridge High School 2012 team.

References